Ppress Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats
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چکیده
Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats Jeffrey M. Lorch, Andrea Gargas, Carol Uphoff Meteyer, Brenda M. Berlowski-Zier, D. Earl Green, Valerie Shearn-Bochsler, Nancy J. Thomas, David S. Blehert From the Molecular and Environmental Toxicology Center, University of Wisconsin– Madison, Medical Sciences Center, Madison, WI (Lorch), the U.S. Geological Survey–National Wildlife Health Center, Madison, WI (Lorch, Meteyer, Berlowski-Zier, Green, Shearn-Bochsler, Thomas, Blehert), and Symbiology LLC, Middleton, WI (Gargas). Corresponding Author: David S. Blehert, U.S. Geological Survey–National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711. [email protected] Running Title: A rapid polymerase chain reaction assay for bat white-nose syndrome
منابع مشابه
Rapid polymerase chain reaction diagnosis of white-nose syndrome in bats.
A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia ...
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